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"centre-name": "Uppsala University",
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"study-abstract": "Sequencing a mock community consisting of T. roseus DSM 18391, C. akajimensis DSM 45221, P. stutzeri DSM 4166, P. inhibens DSM17395, and G. obscurus DSM 43160 for a comparative analysis of multiple displacement amplification performed in standard bulk reactions or in emulsions. Samples also include unamplified controls and no template negative controls.",
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"study-abstract": "To study which genes are specifically induced during the different growth phases in toluene, which could indicate the changes in BTEX metabolism as well as the biological processes affected during the different growth phases, a controlled RNA-seq transcriptome analysis was carried out. Gene expression levels were analyzed from cultures upon inoculation (T0), after 4(T4) and 24(T24) hours after inoculation in minimal medium with toluene.",
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"study-abstract": "Entomopathogenic nematodes from the genus Steinernema are used in biological control of insect pests. The free form of the nematode (infective juvenile) is in closed symbiotic association with the intestinal bacteria Xenorhabdus (Enterobacteriaceae). In the well-studied Steinernema carpocapsae - Xenorhabdus nematophila couple, it was largely shown that the bacterial symbiont played an important role in the death of the insect and in the elimination of the microbial competitors inside the insect cadaver. However, in addition to symbiotic bacteria, other cultivable bacteria have been regularly isolated from infective juveniles nematodes.In this study,we used both culture-dependent and multigenic metabarcoding methods to obtain a comprehensive survey of S. carpocapsae bacterial microbiota. We used two markers, the V3-V4 region of the 16S rRNA gene and a region of the rpoB gene, for which a database was built. We identified a core-microbiota of about 10 OTUs that were present in more than 70% of the samples. Representative bacterial strains of the core-microbiota were tested for their insecticidal performances and their ability to produce antimicrobial molecules.",
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"secondary-accession": "ERP107745",
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